Table 3.
Primers used in this study
| Primer use and primer | Sequence (5′-3′) | Reference |
|---|---|---|
|
Detection
tet
(A) | ||
| Tet(A)-F |
GCTACATCCTGCTTGCCTTC |
[27] |
| Tet(A)-R |
CATAGATCGCCGTGAAGAGG |
[27] |
|
Detection
tet
(B) | ||
| Tet(B)-F |
TTGGTTAGGGGCAAGTTTTG |
[27] |
| Tet(B)-R |
GTAATGGGCCAATAACACCG |
[27] |
|
Detection
tet
(M) | ||
| Tet(M)-1 (266) |
GTTAAATAGTGTTCTTGGAG |
[16] |
| Tet(M)-2 (267) |
CTAAGATATGGCTCTAACAA |
[16] |
|
Detection Tn
916
-like (
xis
-
Tn
) | ||
| Tn916-1 (327) |
GCCATGACCTATCTTATA |
[20] |
| Tn916-2 (328) |
CTAGATTGCGTCCAA |
[20] |
|
Detection Tn
5397
-like (
tndX
) | ||
| Tn5397-tndX-1 (864) |
ATGATGGGTTGGACAAAGA |
[20] |
| Tn5397-tndX-2 (865) |
CTTTGCTCGATAGGCTCTA |
[20] |
|
Detection Tn
5801
-like (
int
) | ||
| intcw459-1 (1811) |
CCGATATTGAGCCTATTGATGTG |
[22] |
| intcw459-2 (1812) |
GTCCATACGTTCCTAAAGTCGTC |
[22] |
|
Amplification and sequencing
tet
(M) | ||
| TetM-upstream (526) |
TTGAATGGAGGAAAATCAC |
[20] |
| TetM-up (323) |
CTGGCAAACAGGTTC |
[20] |
| TetM sequence-1 (525) |
TACTTTCCCTAAGAAAGAAAGT |
[20] |
| TetM sequence-3 (540) |
GCAGAAATCAGTAGAATTGC |
[20] |
| TetM sequence-6 (709) |
TCGAGGTCCGTCTGAAC |
[22] |
| Reverse TetM-2 (307) |
TTGTTAGAGCCATATCTTAG |
[20] |
| TetM sequence-9 (1756) |
AACAGTAAAATGTATAGAGGTG |
[22] |
| F2R (1837) |
GTGTCTTATACCATGGAAGGA |
[22] |
| TetM-down (324) |
TAGCTCATGTTGATGC |
[22] |
| Tet(M)-1 (266) | GTTAAATAGTGTTCTTGGAG | [16] |