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. 2014 Jul 21;9(7):e102700. doi: 10.1371/journal.pone.0102700

Figure 4. mRNA and protein expression of cystine transporters Slc3a1/rBAT and Slc7a9/b0,+AT in kidney cortex from 129S2/SvPasCrl and C57bBL/6J mice.

Figure 4

A–B, Quantitative PCR: Slc3a1 and Slc7a9 mRNA expression was similar in both strains. C, Western Blot: b0,+AT was expressed at a similar level in kidney cortex from both strains. D, Western Blot: rBAT was expressed in C57BL/6 mice but not in 129S2/SvPasCrl mice. E–H, Antibodies directed against the extracellular part of rBAT (Figures 4E and 4G) or against its intracellular part (Figures 4F and 4H) revealed the presence of rBAT at the brush border of proximal tubular cells in C57BL/6J mice (4E and 4F) but not in 129S2/SvPasCrl mice (4G and 4H).