Figure 2. UVA induces CatK expression in a dose-dependent manner.

Cultured fibroblasts were mock irradiated or irradiated with 10/cm² UVA, 20 J/cm² UVA and 30 J/cm² UVA. Cells were harvested 48 h after UVA irradiation for total RNA and protein extraction. (A) Quantitative analysis of CatK mRNA level with real-time RT-PCR. (B) Analysis of CatK protein level by western blot using anti-CatK antibody. Blots were reprobed with anti-GAPDH to confirm equal loading. Bar graphs represent the relative band intensities (mean±SD from three independent experiments). *P<0.05 vs. untreated control, §P<0.05 vs. both other dose-treated groups.