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. 2014 Jul 21;9(7):e102732. doi: 10.1371/journal.pone.0102732

Figure 4. Inhibitory effects of SP600125 and SB203580 on UVA-activated MAPK pathway.

Figure 4

Cells were pretreated with 800 µM SB203580 for 1 h, mock-irradiated or irradiated with 10 J/cm2 UVA, and then incubated in culture medium supplemented with MAPK inhibitors for 1.5 h. Fifteen micrograms of total cellular lysate was electrophoresed on 10% SDS polyacrylamide gels, transferred to a PVDF membrane, and immunodetected using optimal primary and secondary antibody concentrations. Western blot data are representative of three independent experiments. (A) Effects of SP600125 and SB203580 on JNK activity through detection of changes in Jun phosphorylation; (B) Effects of SP600125 and SB203580 on p38 activity through detection of changes in MAPKAPK-2 phosphorylation. *P<0.05 vs. untreated control, §P<0.05 vs.UVA-treated control.