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. 2014 Jul 21;9(7):e102732. doi: 10.1371/journal.pone.0102732

Figure 6. effect of Jun and Fos siRNA on UVA-induced CatK expression.

Figure 6

Cells were cotransfected at 50–70% confluence with 50 nM Jun siRNA and 50 nM Fos siRNA, or 50 nM non-targeting control siRNA (NC) according to the manufacturer's protocol. At 24 h following transfection, the medium was replaced with PBS, cells in control (C), non-targeting-siRNA transfected control (NC) and Jun and Fos siRNA transfected group (siRNA) were not irradiated, whereas cells in UVA-C, UVA-NC and UVA-siRNA were irradiated by 10 J/cm2 UVA, and then incubated with fresh culture medium for an additional 48 h. (A) mRNA level of Jun and Fos in their siRNA-transfected fibroblasts. (B) protein level of Jun and Fos in their siRNA-transfected fibroblasts.(C) Effect of Jun and Fos siRNA on UVA-induced CatK mRNA expression. Total cellular RNA was isolated at 48 h after irradiation, and was analyzed by real-time RT-PCR for CatK. (D) Effect of Jun and Fos siRNA on UVA-induced CatK protein expression. Total cellular protein was harvested at 48 h after irradiation,then was analyzed by Western blot with anti-CatK antibody. *P<0.05 vs. untreated control, §P<0.05 vs. UVA-treated control.