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. 2014 Jul 21;9(7):e102997. doi: 10.1371/journal.pone.0102997

Figure 2. miRNA depleted HT29 cells is not able to support EV71 replication.

Figure 2

DGCR8 knockdown HT29 cells and control HT29 cells (scrambled DsiRNA) were infected with EV71 (MOI of 1). Total intracellular RNA were harvested at 36hpi, converted to cDNA and measured by qPCR with primers specific to viral VP1. Total intracellular protein were harvested at at 36hpi and measured by western blot (A) Relative expression of EV71 viral RNA measured by qPCR. (n = 3, **** = p values of <0.0001) (B) Western blot analysis for EV71 VP1 viral protein. (C) Cell viability assessed at 72hpi using vital dye trypan blue. (n = 3, ** = p values of <0.01).