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. 2014 Jun 27;10:21. doi: 10.1186/1746-4811-10-21

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Copyright © 2014 Healey et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DNA quality and yield assessment for Coffea brassii genomic DNA using the NGS extraction protocol. A) Genomic DNA preparation of Coffea brassii resolved by electrophoresis. 1 kb DNA ladder (1), 100 ng and 200 ng λ DNA standards respectively (3, 4), and DNA extraction using the modified NGS extraction protocol (2). DNA was separated by electrophoresis in a 0.7% agarose gel and visualized using SYBR Safe DNA gel stain. The gel image was cropped to exclude an unrelated sample. B) NanoDrop measurement profile of Coffea brassii leaf extracted genomic DNA using the modified NGS extraction protocol.