Table 2.

Real time PCR analysis of the expression of mRNA for the integrin subunits α _v , β ₃ and β ₅ in HUVEC cultured for 5 h in basal conditions and with VEGF, EGF, IGF, and FGF, alone (control) or in the presence of 1 μM cyclo [DKP-RGD] 1

Subunit	control	+ cyclo [DKP-RGD] 1
	2 -Δct ×10 2	2 -Δct ×10 2	Ratio vs Control	P vs Control
A. Basal conditions
αv	6.65 ± 6.41	6.59 ± 5.24	1.32 ± 0.57	0.944
β3	0.89 ± 0.87	0.91 ± 0.76	1.08 ± 0.27	0.928
β5	1.36 ± 1.18	1.52 ± 1.34	1.08 ± 0.07	0.225
B. With VEGF, EGF, IGF, and FGF
αv	13.31 ± 12.80	15.09 ± 10.66	1.28 ± 0.42	0.494
β3	1.15 ± 1.07	1.41 ± 1.07	1.43 ± 0.35	0.288
β5	1.23 ± 1.25	1.31 ± 1.13	1.34 ± 0.46	0.461

Data are means ± SD of 3 separate experiments.