Figure 5.

HSV-1 VP16 expression. VP16 expression is shown in a logarithmic scale and measured in triplicates from triplicate monolayer cultures. These assays are done from the same samples from which also the TaqMan® -gene expression assays were performed. The log scale shows the normalized VP16 copy number/GAPDH copy number. Mean values are shown + SEM. Testing was done using the Mann–Whitney U-test comparing the irradiated and non-irradiated groups. No statistically significant differences were found between samples. General linear modeling found no significant effect of 2 Gy irradiation on HSV-1 VP16 expression (p = 0.614, R squared = 0.046).