Skip to main content
. 2014 Jul 21;9(7):e102373. doi: 10.1371/journal.pone.0102373

Figure 1. Effect of leptin on TNF-α expression in Raw 264.7 cells.

Figure 1

(A,B) Raw 264.7 cells were treated with leptin (20 nM) for 30 min. Total RNA was isolated using TRIzol reagent, and mRNA levels were determined by semi-quantitative and real-time RT-PCR with primers for TNF-α or GAPDH. *p<0.05 vs control. (C) For ELISA, cells in 96-well culture plates were treated with leptin (20 nM) for the indicated times. Results are the mean ± S.E. for each group of samples. Data are means ± S.E. of eight values. *p<0.05 vs control.