Figure 2. Comparison of Cys and GSH levels in roots and shoots of NT and CdPCS1 expressing rice seedlings.

NT and transgenic lines of rice (Line-1, Line-2 and Line-3) were grown for 10 days on Hewitt media and then transferred in fresh Hewitt media containing 100 μM Na₂AsO₄. Levels of Cys (A) and GSH (B) were measured in root and shoot extracts (after 10 days of treatment) after derivatization with mBBr, separation using HPLC and fluorometric detection. Data are expressed as means ± SD of at least 3 independent experiments. Values marked with similar letters are not significantly (Duncan's test: p < 0.05) different.