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. 2014 Jul 22;4:5784. doi: 10.1038/srep05784

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NT and transgenic lines of rice (Line-1, Line-2 and Line-3) were grown for 10 days on Hewitt media and then transferred in fresh Hewitt media containing 100 μM Na₂AsO₄. After 10 days of treatment, PCS activity in roots (A) and shoots (B) was measured as the production of PC2 min⁻¹ mg⁻¹ protein⁻¹. Levels of PC2 were measured in root and shoot extracts after derivatization with mBBr, separation in HPLC and fluorometric detection. Correlation between PCS activity and accumulation of PCs in root (C) and shoot (D) of NT and CdPCS1expressing rice seedlings. Data are expressed as means ± SD of at least 3 independent experiments. Values marked with similar letters are not significantly (Duncan's test: p < 0.05) different.