Table 1. Influence of duration after nitrogen depletion on Symbiodinium cells.
Normal | Starvation (Day 5) | Starvation (Day 30) | |
---|---|---|---|
Cell size (diameter, μm) | 6.49 ± 1.06a | 6.22 ± 0.88b | 6.49 ± 1.16a |
(n = 688) | (n = 592) | (n = 446) | |
Cell wall (thickness, μm) | 0.02 ± 0.005a | 0.03 ± 0.01b | 0.056 ± 0.02c |
(n = 470) | (n = 994) | (n = 662) | |
Chl a (pg cell−1) | 4.22 ± 0.01b | 4.8 ± 0.01a | 1.93 ± 0.01c |
Lipid droplets size (area, μm2) | 0.38 ± 0.31b | 0.35 ± 0.27b | 1.17 ± 0.77a |
(n = 166) | (n = 200) | (n = 1287) | |
Lipid droplets number in one cells | 2.19 ± 1.54b | 1.63 ± 0.93b | 7.9 ± 2.87a |
(n = 75) | (n = 126) | (n = 163) | |
PAM | 0.44 ± 0.10a | 0.44 ± 0.06a | 0.45 ± 0.05a |
(n = 6) | (n = 10) | (n = 4) | |
Cell density (×106 cells per mg protein) | 15.44 ± 4.18a | 10.02 ± 2.09a | 2.12 ± 0.82b |
(n = 4) | (n = 4) | (n = 4) |
Impact of the nutrient regime on the cell patterns (i.e., Cell size, cell wall, Chl a, Lipid droplets size, Lipid droplets number, PAM, cell density) between the Symbiodinium cells grown in normal and nitrogen-depleted media, and the cell patterns were analyzed by ANOVA. Upper level a–c denote statistical significance different between normal, starvation(day 5) and starvation(day 30), respectively (P<0.001). Data are presented as mean ± SD. Cell wall, Lipid droplets number and cell size: n = number of cell analyzed, Lipid droplets size: n = number of lipid droplets analyzed, PAM and Cell density: n = number of repetition.