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. 2014 Aug;20(8):1210–1222. doi: 10.1261/rna.044529.114

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© 2014 Fung et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Reverse hybrids validate the identified two independent sequence elements for optimal substrate utilization. (A) Cloverleaf structures of tRNA hybrid construct 16–18. (B) A graphical display of the initial rate of product formation vs. tRNA concentration for tRNA^Leu (CAG) (♦), tRNA^Leu (GAG) (Δ), constructs 16 (□), 17 (▪), and 18 (•). Errors represented are the standard deviation of three independent experiments.