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. 2014 Aug;20(8):1238–1247. doi: 10.1261/rna.043141.113

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© 2014 Zhabokritsky et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 6. — Addition of pcRev rescues the HIV-1 splicing phenotype in the presence of PAP. 293T cells were cotransfected with (A) pRev(-) proviral clone (5 μg) or (B) pMenv(-) proviral clone (5 μg) and pcPAP, pcPAPx, or pcDNA3 (2.5 μg), with or without pcRev (2.5 μg). Cells were harvested 40 h post-transfection and Northern blotting was performed on total cellular RNA (15 μg). Levels of the three HIV-1 mRNA classes were visualized by hybridization with the HIV-1-specific riboprobe. Blots were also hybridized with a 5S rRNA-specific riboprobe as a loading control.