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Direct interaction of TbEIF4E5 with two of the five T. brucei EIF4G homologs. Interactions between TbE5 and the five TbEIF4G homologs were challenged using the yeast two-hybrid assay in the presence of increasing amounts of 3AT, increasing the stringency of the assay. Positive controls were pGADT7-T and pGBKT7-53; the negative controls were the empty vectors. Interaction strength is inferred by colony size: ≥2 mm = strong; 1–2 mm = moderate; ≤1 mm = weak.
