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. 2014 Aug;20(8):1272–1286. doi: 10.1261/rna.045534.114

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© 2014 Freire et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — TbEIF4E5 is cytosolic in T. brucei procyclic cells. Subcellular localization was determined by indirect immunofluorescence using antibody against the protein A component of the PTP tag on the TbE5 fusion protein. Nontransfected control YTAT cells served as the negative control. Nuclear and kinetoplast DNA was visualized by counterstaining with DAPI.