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. 2014 Aug;20(8):1272–1286. doi: 10.1261/rna.045534.114

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© 2014 Freire et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — TbEIF4E5 does not have a primary role in translation. (A) Growth of TbE5 RNAi tetracycline (Tet) induced and uninduced cells measured over 7 d. (B) Assessment of protein knockdown by RNAi using the TbE5^PTP/+ background cell line. SDS-PAGE analysis of protein levels at days 2, 3, and 4 post-induction. TbE5-PTP was detected by anti-Protein A antibody (α-ProtA). Serial twofold dilutions of uninduced samples at day 2 are shown for comparison. Levels of TbEIF4A1 are included as protein loading controls. (C) Metabolic labeling of cultures induced for TbE5 RNAi knockdown as measured by ³⁵S-methionine incorporation at 4 and 7 d post-induction.