Fig. 5.

Specific targeting of breast cancer cells using Her2/neu antibody conjugated magnetic nanoparticles (MNPs).

Dual fluorescent imaging using confocal laser scanning microscopy (CLSM) was performed on SKBR-3 breast cancer cells after treatment with bare Lipo(TR)[MNP@m-SiO₂(FITC)] without antibody conjugation and Lipo(TR)[MNP@m-SiO₂(FITC)]-Her2_Ab (A); negative control Lipo(TR)[MNP@m-SiO₂(FITC)]-cetuximab (B) carrying green and red organic dyes were subjected to fluorescence imaging by using CLSM. Uptake of MNPs through endocytosis was hindered by decreasing incubation temperature for SKBR-3 cells from 37℃ to 4℃ (C). Green = FITC, Red = hydrophilic regions, Blue = DAPI/nuclei