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. 2014 Jul 5;10:22. doi: 10.1186/1746-4811-10-22

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Copyright © 2014 Pasin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Laser scanner imaging of fluorescent protein-expressing leaves. Fluorescent proteins were transiently expressed by co-infiltrating N. benthamiana leaf tissue with an Agrobacterium pSN.5 p19 culture plus cultures of Agrobacterium containing pBin-35S-mGFP5 (mGFP5), pSN.5 TagRFP-T (TagRFP-T), pSN.5 mTFP1 (mTFP1) or pSN.5 mPapaya1 (mPapaya1). Fluorescence was imaged by leaf laser scanning. (A) Signal acquired at 6 dpa for TagRFP-T (red) and mGFP5 (green); green and red channel images were merged. Scale bar, 2 cm. (B) Signal at 3 dpa for mTFP1 (cyan) and mPapaya1 (yellow); cyan and yellow channel images were merged. Scale bar, 2 cm. (C,D) Surface plots of infiltrated patches from above images.