Table 1.
Reporter proteins and fluorescence analysis conditions evaluated
|
Reporter |
Laser scanner imaging |
Plate reader FI quantification |
Species |
Structure |
Ref. |
||
|---|---|---|---|---|---|---|---|
| Laser (nm) | Em (nm) | Ex (nm) | Em (nm) | ||||
| mTagBFP2 |
n.a.1 |
n.a. |
400/9 |
455/20 |
Entacmaea quadricolor |
Monomer |
[31] |
| mTFP1 |
457 |
526SP |
450/9 |
480/20 |
Clavularia sp. |
Monomer |
[32] |
| mGFP5 |
488 |
526SP |
485/9 |
535/20 |
Aequorea victoria |
Weak dimer |
[29] |
| sGFP(S65T) |
488 |
526SP |
485/9 |
535/20 |
Aequorea victoria |
Weak dimer |
[30] |
| mNeonGreen |
n.t.2 |
n.t. |
500/9 |
530/20 |
Branchiostoma lanceolatum |
Monomer |
[33] |
| mPapaya1 |
532 |
555/20 |
520/9 |
550/20 |
Zoanthus sp. |
Monomer |
[34] |
| TagRFP-T | 532 | 580/30 | 560/9 | 595/20 | Entacmaea quadricolor | Monomer | [35] |
1n.a., not applicable.
2n.t., not tested.