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. 2014 Jul 12;56(1):42. doi: 10.1186/s13028-014-0042-6

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Copyright © 2014 Stenfeldt et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Myocarditis in two pigs infected with FMDV A24 Cruzeiro (case 1; A-B) or FMDV O1 Campos (case 2; C-D). Both cases have infiltrates of distinct populations of CD8+ and CD163+ cells which are in close proximity to FMDV infected cardiomyocytes but which do not co-localize with FMDV capsid (red) or actin (blue). On serial sections, there was no detection of CD3 and CD21, indicating a CD8+/CD3-/CD21 –phenotype, consistent with NK-cells. FMDV VP1 capsid protein (red), actin (blue), CD163 (turquoise), CD8 (green). Multichannel immunofluorescence, A&C: scale bars =25 μm. B&D: scale bars = 10 μm.