Table 2.
Plasmids used in the current work
| Plasmids | Relevant features | Origin |
|---|---|---|
| p426GPD |
Multicopy URA3 2 μm TDH3p-CYC1t |
(Mumberg et al. [1995]) |
| p426GPD –YAP1 |
TDH3p-YAP1-CYC1t |
This work |
| pUG6 |
kanMX flanked by loxP sites |
(Güldener et al. [1996]) (EUROSCARF; accession number P30114) |
| YE-plac 112 KanR |
Multicopy, KanMX |
(Jeppsson et al. [2003]) |
| YE-plac 112 KanR-YAP1 |
TDH3p-YAP1-CYC1t |
This work |
| pJET1,2 |
Multicopy |
Thermo Scientific, Belgium |
| pJET1,2-attB-KanMX-attP |
Multicopy, KanMX under TEFp |
This work |
| p-intYAP1 |
TDH3p-YAP1-CYC1t – KanMX attB/attP system; integrative |
This work |
| pSTBlue-1 |
multi-purpose cloning vector with dual kanamycin/ampicillin resistance. |
Novagen (EMD Millipore) |
| pSTBlue-YLR446W |
pSTBlue-1 with the YLR446W gene cloned into the multiple cloning region |
This work |
| pYX012 |
Integrative; URA3; TPI1p |
R&D System, Inc., Wiesbaden, D |
| pYX012-LoxPkanMXLoxP |
pYX012, with the KanMX cassette flanked by loxP sites, deriving from pUG6 |
This work |
| pYX012-LoxPKanMXLoxP-MCR1 |
pYX012- LoxPKanMXLoxP with the MCR1 gene inserted in the MCS under the control of the TPI1 promoter |
This work |
| pSTBlue-YLR446WΔ- LoxPKanMXLoxP-MCR1 |
pSTBlue-YLR446W with the LoxPKanMXLoxP-MCR1 cassette, deriving from pYX012 LoxPKanMXLoxP-MCR1, inserted into the YLR446W sequence |
This work |
| pSH65 |
Centromeric plasmid, GAL1p-cre, bler |
(Gueldener et al. [2002]) (EUROSCARF; accession number P30122) |
| pJET1,2- attB-hph- attP |
Multicopy, hph under TEFp |
This work |
| pBEVY-Nat-phiC31 | Multicopy, PhiC31 integrase | This work |