Figure 3.

Modification of UDP (100 μM)-induced increase in the voiding frequency by i.v. application of A317491 (100 nM, a selective P2X3 receptor antagonist) and MRS2179 (0.3 μM, a P2Y₁ receptor antagonist) in the anaesthetized rat. (A) Bladder cystometry recordings during normal saline (0.9%·w/v of NaCl) infusion into the urinary bladder in the absence and in the presence of UDP (100 μM). A317491 (100 nM) and MRS2179 (0.3 μM) were continuously perfused through the catheter inserted into the left jugular vein starting at least 20 min before UDP application. (B) Scatter plots representing the percent change in the ICI and of the duration (Δt) of voiding contractions, compared with control values (Ctr, 0%). The vertical bars represent SEM of a n number of animals (shown in parenthesis). P values as shown; significantly different from UDP alone; unpaired Student's t-test with Welch's correction.