Figure 7.
(A) Comparison of bladder cystometry recordings obtained during infusion of ARL 67156 (100 μM, an E-NTPDase inhibitor) and PSB0474 (100 nM, a selective P2Y6 receptor agonist) into the urinary bladder lumen of anaesthetized rats. (B) Intravesical infusion of PSB0474 (100 nM) increases ATP levels in the urinary fluid collected during cystometry recordings. The ATP content of the samples was quantified by the luciferin-luciferase bioluminescence assay. The vertical bars represent SEM of five animals. *P < 0.05; significantly different from control (saline infusion); one-way anova followed by Dunnett's modified t-test.