Figure 8.

(A) Effect of UDP (100 μM) on electrically-evoked [³H]-ACh release from intact urinary bladder strips and in preparations without the urothelium UDP (100 μM) was applied 8 min before S₂. MRS2578 (50 nM) and MRS2179 (300 nM) were added to the incubation media at the beginning of the release period (time zero) and were present throughout the assay, including S₁ and S₂. The ordinates represent evoked tritium outflow expressed by S₂/S₁ ratios, i.e. the ratio between the evoked [³H]-ACh release during the second period of stimulation (in the presence of UDP) and the evoked [³H]-ACh release during the first stimulation period (without UDP). The vertical bars represent SEM. *P < 0.05; significantly different from control; #P < 0.05: significantly different from UDP alone; unpaired Student's t-test with Welch's correction. (B) Representative microscopic images of rat urinary bladder strips stained with haematoxylin-eosin to confirm the presence or the absence of the urothelium. Magnification, 40×.