Figure 3.
Representative light microscopic images of whole lung cross-sections (upper panels; scale bar = 4 mm) and higher magnification of the same samples (lower panels; scale bar = 200 μm) stained with Martius/Scarlet/Blue (MSB) (A), and quantitative assessment of lung weight (B) and total lung collagen (C) in saline controls, bleomycin (BLEO; 1 mg·kg−1)-treated animals, and mice receiving bleomycin in the absence and presence of sildenafil (SILD; 30 mg·kg−1·day−1), ecadotril (ECAD; 60 mg·kg−1·day−1) or sildenafil plus ecadotril (doses as above). Arrows indicate areas of overt fibrosis. #P < 0.05 versus saline control; *P < 0.05 versus bleomycin. n = 17–25 animals in each group.