Figure 6.

Effect of AEA on Ba²⁺ currents mediated by L-type Ca²⁺ channels, and effects of PTX pretreatment and cannabinoid receptor antagonists on AEA inhibition of L-type Ca²⁺ channels. (A) Traces of normalized Ca²⁺ and Ba²⁺ currents through L-type VGCCS. Normalized Ba²⁺ current in the presence 1 μM AEA was presented in the figure. (B) Effect of 1 μM AEA on the maximal amplitudes and the inactivation kinetics of Ba²⁺ currents. Data shown are means ± SEM; n = 7–8 cells. (C) Percentage of inhibition of L-type Ca²⁺ currents after vehicle (distilled water) and PTX (2 μg·μL⁻¹, 3 h). Data shown are means ± SEM; n = 6–8 cells. (D) Effects of CB1 antagonist AM251 (0.3 μM) and CB2 antagonist AM630 (0.3 μM) on AEA (1 μM) inhibition of L-type Ca²⁺ currents. Data shown are means ± SEM; n = 6–9 cells. *P < 0.05, significantly different from control.