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(A) Plotting of competitive binding of ApoG2 and (-)-gossypol to purified recombinant Bcl-2 protein. (B) Treatment of WSU-DLCL2 with ApoG2 interferes with the formation of heterodimers between Bcl-XL and Bim. Co-immunoprecipitation with anti-Bcl-XL antibody; Western blot with anti-Bim and anti-Bcl-XL antibodies. (C) Western blot of whole cell lysate with anti-Bim and anti-Bcl-XL antibodies. β-actin was probed to show loading of equal amount of protein lysate.
