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. Author manuscript; available in PMC: 2014 Jul 22.
Published in final edited form as: Nat Neurosci. 2013 Sep 1;16(10):1401–1408. doi: 10.1038/nn.3505

Figure 3.

Figure 3

Ablation of Act1 in oligodendrocyte lineage ameliorates autoimmune encephalomyelitis. (a) Immunoblot analysis for the Act1 expression in cultured NG2+ OPCs from embryonic brains. Full-length blots are presented in Supplementary Figure 5. (b) Mean clinical score of EAE in NG2CreAct1fl/− and NG2CreAct1fl/+ mice induced by active immunization with MOG35–55(p<0.05, two-way ANOVA). (c) Mean clinical score of EAE in NG2CreAct1fl/− and NG2CreAct1fl/+ mice induced by Th1 adoptive transfer (p>0.05, two-way ANOVA). (d) Mean clinical score of EAE in NG2CreAct1fl/− and NG2CreAct1fl/+ mice induced by Th17 adoptive transfer (p<0.05, two-way ANOVA). (e) H&E, Luxol fast blue, anti-CD11b staining of lumber spinal cords of NG2CreAct1fl/− and NG2CreAct1fl/+ mice 20 days after Th17 adoptive transfer. Data are representative of 5 mice per group. (f) Absolute cell numbers of infiltrated immune cells in the brains of NG2CreAct1fl/− and NG2CreAct1fl/+ mice (20 days after Th17 cell transfer). (g) Real-time PCR analysis of inflammatory gene expression in spinal cords of NG2CreAct1fl/− and NG2CreAct1fl/+ mice transferred with MOG35–55-specific Th17 cells. Data are representative of three independent experiments. n=5/group in each experiment. Error bars, SEM; *, p<0.05.