Table 1.
Primer sequences for PCR analysis.
Gene | Forward primer | Reverse primer |
---|---|---|
Total PGC-1α | 5′-TGC CAT TGT TAA GAC CGA G-3′ | 5′-GGT CAT TTG GTG ACT CTG G-3′ |
Total NT-PGC-1α | 5′-TGC CAT TGT TAA GAC CGA G-3′ | 5′-GGT CAC TGG AAG ATA TGG C-3′ |
NT-PGC-1α-a | 5′-TTG ACT GGC GTC ATT CG-3′ | 5′-CTG GAA GAT ATG GCA CAT-3′ |
NT-PGC-1α-b | 5′-CAT GGA TTC AAT TTT GAA ATG-3′ | 5′-GGT CAC TGG AAG ATA TGG C-3′ |
NT-PGC-1α-c | 5′-CTA TGC TGC TGT GTG CTG-3′ | 5′-GGT CAC TGG AAG ATA TGG C-3′ |
PGC-1α-a | 5′-TTG ACT GGC GTC ATT CG-3′ | 5′-GGT CAT TTG GTG ACT CTG G-3′ |
PGC-1α-b | 5′-CAT GGA TTC AAT TTT GAA ATG-3′ | 5′-GGT CAT TTG GTG ACT CTG G-3′ |
PGC-1α-c | 5′-CTA TGC TGC TGT GTG CTG-3′ | 5′-GGT CAT TTG GTG ACT CTG G-3′ |
Glut4 | 5′-GTT GGT CTC GGT GCT CTT AGT-3′ | 5′-ATA GCA TCC GCA ACA TAC TGG-3′ |
ATP5B | 5′-GGC ACT GAA GGC TTG GTT AG-3′ | 5′-CAA GAG AAG ATT CTC AGC GAC-3′ |
Cyc1 | 5′-CCC TGA CCT CAG CTA CAT C-3′ | 5′-CAA GAG AAG ATT CTC AGC GAC-3′ |
COX5B | 5′-GCT GCA TCT GTG AAG AGG ACA AC-3′ | 5′-CAG CTT GTA ATG GGT TCC ACA GT-3′ |
MHC I | 5′-CCA AGG GCC TGA ATG AGG AG-3′ | 5′-GCA AAG GCT CCA GGT CTG AG-3′ |
MHC II a | 5′-AAG CGA AGA GTA AGG CTG TC-3′ | 5′-GTG ATT GCT TGC AAA GGA AC-3′ |
MHC II b | 5′-CGA AGG CGG AGC TAC GGT CA-3′ | 5′-CGG CAG CCA CTT GTA GGG GT-3′ |
MHC II x | 5′-GCC AGG GTC CGT GAA CTT GAA G-3′ | 5′-CCT CCG CTT CCT CAG CTT GTC T-3′ |
IGF-1 | 5′-AAA GCA GCC CGC TCT ATC C-3′ | 5′-CTT CTG AGT CTT GGG CAT GTC A-3′ |
Myostatin | 5′-GGC CAT GAT CTT GCT GTA AC-3′ | 5′-TTG GGT GCG ATA ATC CAG TC-3′ |
Cyclophilin | 5′-CCA TCG TGT CAT CAA GGA CTT CAT-3′ | 5′-CTT GCC ATC CAG CCA GGA GGT CTT-3′ |
PGC-1α: peroxisome proliferators-activated receptor-γ coactivator-1α; NT-PGC-1α: N-truncated PGC-1α; Glut4: glucose transporter 4; ATP5B: ATP synthase B subunit; Cyc1: cytochrome C unit 1; COX5B: cytochrome oxidase 5 B subunit; MHC I: myosin heavy chain I; MHC II a: myosin heavy chain II a; MHC II b: myosin heavy chain II b; MHC II x: myosin heavy chain II x; Cyclophilin B: peptidylprolyl isomerase B; IFG-1: insulin-like growth factor 1.