. 2014 Jul 3;2014:578698. doi: 10.1155/2014/578698

Table 1.

Approximate diameter (D _c), average volume (V _c), and number of autophagic elements (N _c) in a cell with 5000 μm³ cytoplasm, calculated from the specific surface values (S _v /V _v = S _sp) from real experimental samples of liver and exocrine pancreatic cells. The details of calculation are described in the text.

Experimental system and treatment	Category	S _v μm²/μm³	V _v μm³/μm³	S _sp μm²/μm³	D _c μm	V _c μm³	N _c in 5000 μm³
Isolated hepatocytes
Plus amino acid mixture 30 min	Afs	0,0023	0,0003	7,6	0,79	0,26	6
Plus amino acid mixture 30 min	Al	0,0147	0,0022	6,8	0,88	0,36	30
Plus amino acid mixture 3 h	Afs	0,0047	0,0006	7,4	0,81	0,28	11
Plus amino acid mixture 3 h	Al	0,0367	0,0072	5,1	1,18	0,85	42
Minus amino acid mixture 3 h 30 min	Afs	0,0472	0,0067	7,0	0,86	0,33	102
Minus amino acid mixture 3 h 30 min	Al	0,1333	0,0215	5,9	0,97	0,47	195
Propylamine 10 mM 3 h	Afs	0,0856	0,0124	6,9	0,87	0,34	180
	Al	0,0563	0,0084	6,7	0,90	0,38	112
	Alam	0,2925	0,1170	2,5	2,40	7,24	81

Hepatocytes in vivo
3 h feeding	Afs	0,0024	0,0003	7,9	0,76	0,23	7
	Al	0,0040	0,0005	7,9	0,76	0,23	11
	Db	0,0088	0,0008	11,0	0,55	0,08	47
24 h fasting	Afs	0,0101	0,0014	7,2	0,84	0,31	23
	Al	0,0171	0,0022	7,8	0,77	0,24	46
	Db	0,0079	0,0008	9,9	0,61	0,12	34
Ad libitum feeding	Afs	0,0025	0,0003	8,5	0,71	0,18	8
Ad libitum feeding	Al	0,0048	0,0006	8,0	0,75	0,22	14
Vinblastine treatment 0,1 mg/g 2 h	Afs	0,0778	0,0105	7,4	0,81	0,28	189
Vinblastine treatment 0,1 mg/g 2 h	Al	0,0648	0,0086	7,5	0,80	0,26	163
Leupeptin treatment 0,12 mg/g 2 h	Afs	0,0450	0,0054	8,3	0,72	0,20	138
Leupeptin treatment 0,12 mg/g 2 h	Al	0,1095	0,0278	3,9	1,52	1,85	75

Exocrine pancreas cells in vivo
24 h fasting	Afs	0,0050	0,0007	7,2	0,83	0,30	12
24 h fasting	Al	0,0061	0,0009	6,8	0,89	0,36	12
Ad libitum feeding	Afs	0,0016	0,0002	7,9	0,76	0,23	4
Ad libitum feeding	Al	0,0024	0,0003	7,8	0,77	0,23	6
Vinblastine treatment 0,1 mg/g 1 h	Afs	0,0447	0,0063	7,1	0,85	0,32	99
Vinblastine treatment 0,1 mg/g 1 h	Al	0,0233	0,0034	6,8	0,88	0,35	48
Vinblastine treatment 0,1 mg/g 1,5 h	Afs	0,1073	0,0159	6,8	0,89	0,37	216
Vinblastine treatment 0,1 mg/g 1,5 h	Al	0,0401	0,0060	6,7	0,90	0,38	79
Vinblastine treatment 0,1 mg/g 6 h	Afs	0,3362	0,0518	6,5	0,92	0,41	626
Vinblastine treatment 0,1 mg/g 6 h	Al	0,3081	0,0517	6,0	1,01	0,53	484

Afs: autophagosome (early autophagic vacuole), Al: autolysosome (late autophagic vacuole), Db: dense body, Alam: swollen electron-lucent amine type of autolysosome.

The experimental animals were from male mice for in vivo treatments and from rats for isolated cells. The evaluated cytoplasmic area was in the range of 9–16000 μm². For further details of electron microscopy, morphometry, and experimental systems see, for example, in [5–10].