Figure 2. Patients treated with dasatinib have increased proportions of memory CD4+ T-cells. The memory cell subsets of CD4+ and CD8+ T-cells were examined by staining the fresh or frozen PBMNCs with α-CD45, α-CD3, α-CD4-, α-CD45RA and α-CCR7-PE antibodies after which they were analyzed by flow cytometry. The proportion of (A) naïve (CCR7+CD45RA+), (B) CD45RA+ effector memory (TEMRA, CCR7negCD45RA+) (C) central memory (TCM, CCR7+CD45RAneg) and (D) effector memory TEM (CCR7negCD45RAneg) CD4+ T-cells from the whole CD4+ T-cell population was measured from healthy (n = 7), untreated CML patients (n = 9) and patients treated with dasatinib (DA, n = 6), imatinib (IM, n = 5) or nilotinib (NI, n = 5) at least 12 mo after start of treatment. Same analysis was performed on CD8+ T-cells (E–H). Statistical analyses in all panels were done by 1way ANOVA.