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. 2014 Jul 3;2014:753483. doi: 10.1155/2014/753483

Figure 5.

Figure 5

CTM reduced IL-13 expression profile in the mice liver during Sj infection. Mice are sacrificed at week 8 after Sj infection and their liver or sera are collected. (a) IL-13 mRNA level in mouse liver of normal or Sj-infected mice with or without CTM treatment is detected by RT-PCR and Q-PCR. Upper panel: Q-PCR; lower panel: RT-PCR. GAPDH is detected as an internal control. Data are presented as mean ± SD from eight mice per group. **P < 0.01. (b) IL-13 concentration in mouse sera of normal or Sj-infected mice with or without CTM treatment is detected through ELISA. Data are presented as mean ± SD from 8 mice per group. *P < 0.05. (c) Mouse livers at indicated time points are fixed in paraformaldehyde, embedded in paraffin, sliced, and IHC stained for IL-13. Representative slices of IHC staining for IL-13 are shown at original 40x magnification.