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. 2014 Jul 22;5:228. doi: 10.3389/fgene.2014.00228

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Copyright © 2014 Liu, Fan, Li, Song, Li, Zhang and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PhS_3L-SLF1 and PhSSK1 are both located in the cytosol of pollen grains and pollen tubes. (A) Immunogold labeling of PhS_3L-SLF1::FLAG in pollen grain (left) and pollen tube (right). (B) Immunogold labeling of PhSSK1 in pollen grain (left) and pollen tube (right). PG, pollen grain; Ex, exine; In, intine (arrow); C, cytosol; V, vacuole; PT, pollen tube; PTW, pollen tube wall; TT, transmitting tract tissue. (C) Western blot detection of PhS_3L-SLF1::FLAG (SLF) and PhSSK1 (SSK1) in subcellular fractions of pollen grains. (D) Western blot detection of PhS_3L-SLF1::FLAG (SLF) and PhSSK1 (SSK1) in subcellular fractions of in vitro germinated pollen tubes. WT EC and EC denote entire cell homogenates from wild-type and the transgenic pollen grains or pollen tubes, respectively. Pellet fractions (P1, P12, P40, and P160) and supernatant fractions (S1, S12, S40, and S160) are derived from differential centrifugation at 1000, 12,000, 40,000, and 160,000 g, respectively. cFBP, Sar1, and Arf1 are marker antibodies for cytosol, endoplasmic reticulum (ER) and Golgi, respectively.