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. Author manuscript; available in PMC: 2014 Jul 22.
Published in final edited form as: J Am Chem Soc. 2008 Jul 1;130(30):9836–9843. doi: 10.1021/ja801662y

Table 3.

Copolymers

(a) Precursor polymer characterization by GPCa
sample monomers Mn Target g mol−1 GPC Mn g mol−1 Mw/Mn
E1:P9 ethyl-propyl 3500 11 600 1.08
E1:P2 ethyl-propyl 3400 7000 1.34
E1:P1 ethyl-propyl 3000 11 000 1.08
E2:P1 ethyl-propyl 3500 10 200 1.15
E9:P1 ethyl-propyl 3400 7600 1.30
M9:E1 methyl-ethyl 3400 13 600 1.10
M1:E1 methyl-ethyl 3500 14 400 1.08
M1:E9 methyl-ethyl 3500 14 500 1.08
M9:P1 methyl-propyl 3400 15 300 1.07
M1:P1 methyl-propyl 3600 15 900 1.07
M1:P9 methyl-propyl 3700 14 700 1.07
(b) Characterization of the antimicrobial copolymers by biological assaysb
sample monomer Mn. Target g mol−1 MIC90 μgmL−1
HC50 μg mL−1 selectivty
E. coli S. aureus E. coli S. aureus
Propyl_3k propyl 3000 6.25 15 50 8.0 3.3
E1:P9 ethyl:propyl 3500 15 15 <50 <3.3 <3.3
E1:P2 ethyl:propyl 3400 15 15 <50 <3.3 <3.3
E1:P1 ethyl:propyl 3000 100 200 1000 10 5.0
E2:P1 ethyl:propyl 3500 >200 200 >2000 10 >10
E9:P1 ethyl:propyl 3400 >200 50 500 <2.5 10
Ethyl_3k ethyl 3000 50 50 1400 28 28
Methyl_3k methyl 3000 >200 100 2000 <10 20
M9:E1 methyl-ethyl 3400 >200 6.25 700 <3.5 112
M1:E1 methyl-ethyl 3500 >200 12.5 1200 <6.0 96
M1:E9 methyl-ethyl 3500 >200 12.5 1500 <7 120
Ethyl_3k ethyl 3000 50 50 1400 28 28
Methyl_3k methyl 3000 >200 100 2000 10 20
M9:P1 methyl-propyl 3400 >200 <3.75 >2000 10 >533
M1:P1 methyl-propyl 3600 >200 <3.75 >2000 10 >533
M1:P9 methyl-propyl 3700 >200 <3.75 >2000 10 >533
Propyl_3k propyl 3000 6.25 15 50 8.3 3.3
a

DMF, 0.01 mol L−1 LiCl, PS standards.

b

Inhibitory activity towards bacterial growth of E. coli and S. aureus bacteria (MIC90 = minimal inhibitory concentration preventing 90% bacterial growth) and hemolytic activity towards red blood cells (HC50 = concentration lysing 50% of blood cells). Sample labeling: e.g., E1:P9 is a copolymer with 10 mol% ethyl and 90 mol% propyl monomer.