FIGURE 1.

Fluorescence imaging of affinity-purified cross-linked products. NaCl-washed PSII membranes were incubated with 6.25 mm EDC and 5 mm sulfo-NHS in either the presence (+) or the absence (−) of biotinylated-PsbP (left) or-PsbQ (right). Cross-linked products were purified with Strep-Tactin Sepharose, and the purified peptides corresponding to 2 μg of Chl were separated by SDS-PAGE. The proteins were stained with Flamingo gel stain and visualized with a fluorescence gel imager. Arrows indicate the bands of noncross-linked proteins, and asterisks indicate the bands of unrelated proteins from the resin.