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Immunodetection of affinity-purified cross-linked products. The affinity-purified cross-linked peptides using biotinylated PsbP (A) or PsbQ (B) corresponding to 2 μg of Chl were separated by SDS-PAGE and immunodetected with antisera against CP43, CP26, PsbP, PsbR, and PsbE, or PsbQ, as indicated on each lane. Arrows indicate the protein bands corresponding to P-1, P-2, P-3, P-4, Q-3 and Q-4, respectively.
