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Silencing effects of siRNAs against MMPs. A, aHSCs were treated with siRNA against MMP2, MMP9, MMP12, MMP23, and MT1-MMP at 2, 5, and 10 nm in DMEM containing 10% FBS for 48 h. Suppression of MMP expression was quantitated by quantitative RT-PCR. B, aHSCs were treated with siRNAMT1-MMP at 2, 5, and 10 nm in DMEM containing 10% FBS for 72 h. Expression of MT1-MMP in the cells was analyzed by Western blotting.
