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Silencing effects of integrin siRNAs. A, aHSCs were treated with siRNA against integrin α11, β5, α8, and α5 at 2, 5, and 10 nm in DMEM containing 10% FBS for 48 h. The expression of integrin in those cells was quantitated by quantitative RT-PCR. B, expression of integrin in aHSCs treated with siRNA against integrin α1, α2, αV, β1, and β3 was analyzed by Western blotting.
