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. 2014 May 29;289(29):20307–20317. doi: 10.1074/jbc.M114.576249

FIGURE 6.

FIGURE 6.

Induction of IEGs by FL Reelin. Cortical neurons were treated with FL or CF Reelin for 1 h and mRNA was extracted for quantitative RT-PCR. A, in 4–5 DIV cultures FL Reelin induced Arc mRNA expression in a concentration-dependent manner (10–100 nm), whereas CF Reelin did not. The immunoblot shown below demonstrates induction of Arc protein expression after 3 h of stimulation with higher concentrations of FL, but not CF Reelin. B, FL Reelin induces Egr family of transcription factors, but not c-Fos in 4–5 DIV cortical neurons. CF Reelin has no effect. C, time course of Arc and Egr1–3 mRNA expression by FL Reelin 50 nm. D, in 10–12 DIV neurons, FL Reelin induced the expression of all IEGs examined. *, p < 0.05; **, p < 0.01. ***, p < 0.001.