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. Author manuscript; available in PMC: 2014 Jul 22.
Published in final edited form as: Gene. 2010 Mar 3;458(0):1–10. doi: 10.1016/j.gene.2010.02.006

FIG. 6. Western blot assay of the recombinant Flavobacterium strains expressing the larvicidal genes.

FIG. 6

(A). lane 1, proteins were extracted from F. johnsoniae carrying pSCH175 (p20); lane 2, proteins were extracted from F. johnsoniae carrying pSCH143 (negative control); anti-P20 antiserum was added. (B). lane1, proteins were extracted from E. coli strain carrying pMMB736 (positive control); lane2, proteins were extracted from F. johnsoniae carrying pSCH210 (cry11A); anti-Cry11A was added. (C). lane 1, proteins were extracted from F. johnsoniae carrying plasmid pSCH144 (negative control); lane2, proteins were extracted from F. johnsoniae carrying plasmid pSCH512 (gfp::P42); lane 3, proteins were extracted from F. johnsoniae carrying plasmid pSCH532 (gfp::p51); lane 4. proteins were extracted from F. johnsoniae carrying pFj29; anti-GFP antiserum was used.