FIG. 5.

Increased NY-ESO-1 expression in thyroid cell lines with the DAC treatment allows recognition by NY-ESO-1-specific T-cell lymphocytes. One hundred thousand thyroid cancer cells were treated with DAC and then co-cultured with an equal number of NY-ESO-1 specific T-cell receptor (TCR)-transduced T lymphocytes for 24 hours. DAC treatment of TPC-1 and BCPAP resulted in a significant increase in (A) interferon-gamma (IFN-γ) (2.4- to 2.8-fold) and (B) granzyme-B levels (∼1.4- to 1.5-fold) in the co-culture media when compared with untreated controls. Although 8505c cells show a mild increase in NY-ESO-1 mRNA and protein, it appears not to be significant enough to result in T-cell activation. *P<0.05 versus untreated control. GFP, green fluorescent protein; PBL, peripheral blood lymphocyte.