Fig. 3.

NES1 as a repressor in nitric oxide-induced cotyledon senescence. (A) Relative transcriptional quantification of NES1 in transgenic plants. Seedlings treated as in Fig. 1E. (B) The NES1-overexpressing seedlings (35S::NES1) treated with 40 μM nitroprusside. (C) Chlorophyll content at 72h of plants treated as in Fig. 1A. Data are mean values of 20 seedlings ±SE, with three replicates for each sample. (D) Complementation of the transgenic plants treated as in Fig. 1A. Asterisks indicate significant differences (*P < 0.05; **P ≤ 0.01).