Fig. 4.

Altered expression of SAGs in atg5 leaves under mild and strong abiotic-stress conditions. First-strand cDNAs were prepared from total RNA extracted from 3-week-old rosette leaves of WT and atg5 plants before (control) and after 3 d of mild abiotic-stress treatments (A–D) and strong abiotic-stress treatments (E–H). By RT-qPCR analysis, relative expression levels of SGR1/NYE1 (A, E), NYC1 (B, F), WRKY22 (C, G), and ORE1 (D, H) were obtained by normalizing to the mRNA levels of GAPDH. Mean and SD values were obtained from more than three biological replicates. These experiments were replicated at least twice with similar results. Student’s t-test (*P<0.05, **P<0.01).