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. Author manuscript; available in PMC: 2014 Jul 22.
Published in final edited form as: SOJ Immunol. 2014 Jan 20;2(1):07. doi: 10.15226/soji.2014.00107

Figure 1.

Figure 1

(A) Twenty mice (n = 8 WT, 12 KIKO) were sacrificed at 18 weeks. Spleens from KIKO mice were smaller and there was a significant difference in spleen cell counts between WT and KIKO mice. In a subset of animals (n = 4 WT, 5 KIKO), femurs were harvested and bone marrow cells were counted. There was a trend toward reduced numbers in the KIKO mice that did not reach significance. (B) Ex vivo spleen cells (n = 4 WT, 7 KIKO) and cultured BM-DCs (n = 4 WT, 5 KIKO) were stimulated overnight (18h) with 200umol loxoribine (TLR 7 ligand) or 1ug/mL CpG DNA (TLR9 ligand) under estrogen-free media conditions. TLR stimulation resulted in increased IL-6 production that was significantly decreased in media from KIKO mice compared to wild-type mice.