Figure 12. Dose Response Dependence of WC 21 for the Attenuation of Total AIM Scores.

A. Determination of IC50 value: Unilaterally lesioned rats were injected (i.p.) with varying doses of WC 21 (0 to 10 mg/kg) followed immediately with a constant dose of L-dopa and benserazide (8 mg/kg each). The percent of the mean total AIMs score (n ≥ 8) relative to vehicle controls as a function of the dose of WC 21 is shown. The mean values ± S.E.M. for the normalized total AIMs values as a function of WC 21 concentrations are as follows: a) 1 mg/kg, 81.0 ± 7.7, b) 3.0 mg/kg, 51.2 ± 9.7, c) 6 mg/kg, 24.9 ± 7.0 and d) 10 mg/kg, 1.8 ± 1.5. The calculated IC₅₀ value for this data was found to be 3.8 mg/kg using a linear regression analysis (r² = 0.96). B. Temporal plot: The variation in the total AIMs score as a function of time is shown using zero time pretreatment with i.p. injection of WC 21 at 10 mg/kg (□), 6 mg/kg (▲), 3 mg/kg (■), 1 mg/kg (●) or vehicle control (❍). Animals were also injected (i.p.) with 8 mg/kg L-dopa and benserazide. Each point is the summation of total AIMs score for a total of 8 animals at each observation time point for each dose of WC 21. The temporal plot for the vehicle control curve is the mean value for all four experiments.