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. Author manuscript; available in PMC: 2014 Sep 6.
Published in final edited form as: Nature. 2014 Jan 29;507(7490):62–67. doi: 10.1038/nature13011

Figure 3. Cas9:RNA searches for PAMs and unwinds dsDNA in a directional manner.

Figure 3

a, Schematic of the competition cleavage assay. b, Cleavage assay with and without competitor DNAs. c, Quantitation of competition data (mean ± s.d.). Competitor cartoon representations show PAMs (yellow) and regions complementary to the crRNA (red). d, Predicted data trends for the random nucleation or sequential unwinding models aligned with the corresponding data in (e). e, Competition assays using substrates with variable degrees of crRNA complementarity, shown as in (c). Numeric descriptions of the competitor DNAs indicate the regions of complementarity (red) or mismatches (black) to the crRNA sequence.