Table I.
Metabolite differences between native Streptococcus suis type 2 and CcpA mutant strains.
| LC-MS | Retention time, min | Molecular mass | Metabolite | VIP scorea | Fold changeb |
|---|---|---|---|---|---|
| ESI+ | 0.66 | 103.0631 | γ-aminobutryic acid | 1.98 | 0.58 |
| 0.68 | 165.0456 | S-oxide | 1.55 | 0.29 | |
| 0.68 | 147.0527 | L-glutamate | 1.98 | 0.42 | |
| 0.74 | 159.1260 | DL-2-aminooctanoic acid | 1.28 | −0.45 | |
| 0.74 | 453.2959 | Glycerophospho-N-palmitoyl ethanolamine | 1.83 | −2.38 | |
| 0.82 | 149.0509 | L-methionine | 1.84 | 1.63 | |
| 1.02 | 244.0703 | Uridine | 1.91 | −1.56 | |
| 1.08 | 267.0971 | Deoxyguanosine | 2.29 | 3.20 | |
| 1.16 | 573.1530 | Biotinyl-5′-AMP | 1.74 | −2.28 | |
| 1.17 | 268.0795 | Inosine | 1.67 | −0.98 | |
| 3.72 | 441.2594 | Leukotriene E3 | 1.28 | −0.61 | |
| 10.14 | 255.2567 | Palmitic amide | 1.73 | −0.63 | |
| 10.65 | 329.2575 | 4,8-Dimethylnonanoyl-carnitine | 2.24 | 0.95 | |
| 13.44 | 281.2723 | Oleamide | 1.88 | 0.54 | |
| 13.93 | 160.0887 | Homoglutamine | 1.77 | 0.35 | |
| 15.26 | 384.2865 | 17,20-Dimethyl prostaglandin F1α | 1.84 | 0.76 | |
| 16.07 | 148.0376 | 2-Hydroxyglutarate | 2.21 | 0.88 | |
| ESI− | 0.67 | 133.0378 | L-aspartic acid | 1.76 | 0.32 |
| 0.68 | 147.0535 | L-glutamate | 1.63 | 0.44 | |
| 0.70 | 183.0656 | Phosphocholine | 1.82 | 1.19 | |
| 0.74 | 151.0504 | Guanine | 1.77 | 1.42 | |
| 0.75 | 324.0371 | Uridine monophosphate | 1.95 | 1.89 | |
| 1.00 | 168.0285 | Uric acid | 1.36 | −0.97 | |
| 1.00 | 152.0336 | Xanthine | 1.46 | 0.36 | |
| 1.02 | 244.0707 | Uridine | 1.56 | −0.58 | |
| 1.05 | 192.0272 | Citric acid | 1.64 | −2.85 | |
| 1.16 | 268.0820 | Inosine | 1.67 | −1.86 | |
| 1.17 | 118.0266 | Succinic acid | 1.52 | −0.15 | |
| 1.23 | 131.0948 | L-leucine | 1.47 | −0.82 | |
| 3.80 | 204.0905 | L-tryptophan | 1.53 | −0.25 | |
| 3.93 | 427.2449 | 20-Hydroxy N-arachidonoyl taurine | 1.33 | −0.22 | |
| 4.80 | 643.3191 | Gsh-prostaglandin A1 | 1.86 | −0.32 | |
| 4.93 | 723.3088 | Trypanothione | 1.52 | −0.71 | |
| 5.62 | 187.1212 | 8-Amino-7-oxononanoic acid | 1.35 | 0.68 | |
| 5.83 | 193.0742 | Phenylacetylglycine | 1.61 | −1.14 | |
| 11.72 | 450.1830 | Geranylgeranyl PP | 1.54 | 0.73 | |
| 14.44 | 166.0484 | 7-Methylxanthine | 1.53 | 1.53 |
a
VIP, variable importance in the projection (P<0.05).
b
Fold change was calculated as binary logarithm of average mass response ratio between native Streptococcus suis type 2 and CcpA mutant strain, where the positive value means that the native Streptococcus suis type 2 of the metabolite is larger than the CcpA mutant strain.
CcpA, catabolite control protein A; LC-MS, liquid chromatography-mass spectrometry; ESI, electrospray ionization.