Table I.
Oligonucleotide primers for phosphatase and tensin homologue analysis.
Exon | Forward (5′-3′) | Reverse (5′-3′) | Annealing temp. (°C) | Product size (bp) |
---|---|---|---|---|
1 | TCCTCCTTTTTCTTCAGCCAC | GAAAGGTAAAGAGGAGCAGCC | 56 | 147 |
2 | TGCATATTTCAGATATTTCTTTCCTT | TTTGAAATAGAAAATCAAAGCATTC | 57 | 155 |
3 | TGTTAATGGTGGCTTTTTG | GCAAGCATACAAATAAGAAAAC | 56 | 114 |
4 | TTCCTAAGTGCAAAAGATAAC | TACAGTCTATCGGGTTTAAGT | 56 | 147 |
5 | TTTTTTTTTCTTATTCTGAGGTTATC | GAAGAGGAAAGGAAAAACATC | 51 | 312 |
6 | AGTGAAATAACTATAATGGAACA | GAAGGATGAGAATTTCAAGC | 54 | 231 |
7 | ATCGTTTTTGACAGTTTG | TCCCAATGAAAGTAAAGTAGA | 55 | 262 |
8 | AGGTGACAGATTTTCTTTTTTA | TAGCTGTACTCCTAGAATTA | 52 | 394 |
9 | GTTCATCTGCAAAATGGA | TGGTAATCTGACACAATGTCCTA | 50 | 397 |
bp, base pair.